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細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)培(pei)養(yang)(yang)(yang)（cell culture）是(shi)(shi)指在體外(wai)模擬體內環(huan)境（無(wu)菌、適(shi)宜溫度(du)、酸堿度(du)和一(yi)定(ding)營養(yang)(yang)(yang)條件等(deng)），使之生(sheng)存(cun)、生(sheng)長、繁殖并(bing)維持(chi)主要結構(gou)和功(gong)能的(de)(de)一(yi)種(zhong)方(fang)法(fa)。細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)培(pei)養(yang)(yang)(yang)也(ye)叫細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)克(ke)隆(long)技(ji)(ji)術(shu)(shu)，在生(sheng)物(wu)學中(zhong)的(de)(de)正規(gui)名(ming)詞(ci)為(wei)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)培(pei)養(yang)(yang)(yang)技(ji)(ji)術(shu)(shu)。不(bu)論對于整個(ge)生(sheng)物(wu)工程(cheng)技(ji)(ji)術(shu)(shu)，還是(shi)(shi)其中(zhong)之一(yi)的(de)(de)生(sheng)物(wu)克(ke)隆(long)技(ji)(ji)術(shu)(shu)來說，細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)培(pei)養(yang)(yang)(yang)都是(shi)(shi)一(yi)個(ge)必(bi)(bi)不(bu)可(ke)(ke)少(shao)的(de)(de)過(guo)程(cheng)，細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)培(pei)養(yang)(yang)(yang)本身(shen)就是(shi)(shi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)的(de)(de)大規(gui)模克(ke)隆(long)。細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)培(pei)養(yang)(yang)(yang)技(ji)(ji)術(shu)(shu)可(ke)(ke)以(yi)(yi)由一(yi)個(ge)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)經過(guo)大量培(pei)養(yang)(yang)(yang)成(cheng)為(wei)簡單的(de)(de)單細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)或極少(shao)分化的(de)(de)多細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)，這是(shi)(shi)克(ke)隆(long)技(ji)(ji)術(shu)(shu)必(bi)(bi)不(bu)可(ke)(ke)少(shao)的(de)(de)環(huan)節，而且細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)培(pei)養(yang)(yang)(yang)本身(shen)就是(shi)(shi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)的(de)(de)克(ke)隆(long)。細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)培(pei)養(yang)(yang)(yang)技(ji)(ji)術(shu)(shu)是(shi)(shi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)生(sheng)物(wu)學研究方(fang)法(fa)中(zhong)重(zhong)要和常用技(ji)(ji)術(shu)(shu)，通(tong)過(guo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)培(pei)養(yang)(yang)(yang)既可(ke)(ke)以(yi)(yi)獲得大量細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)，又可(ke)(ke)以(yi)(yi)借此研究細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)的(de)(de)信號轉導、細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)的(de)(de)合(he)成(cheng)代謝、細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)的(de)(de)生(sheng)長增殖等(deng)。